SL327

• CAT Number: I003073
• CAS Number: 305350-87-2
• Molecular Formula: C₁₆H₁₂F₃N₃S
• Molecular Weight: 335.4
• Purity: ≥95%
• Target: MEK1/2
• Solubility: DMSO 25 mg/mL Ethanol 25 mg/mL
• Storage: 3 years -20C powder
• IC50: 0.18/0.22 uM(MEK1/2)
• InChI: InChI=1S/C16H12F3N3S/c17-16(18,19)14-4-2-1-3-12(14)13(9-20)15(22)23-11-7-5-10(21)6-8-11/h1-8H,21-22H2/b15-13+
• InChIKey: JLOXTZFYJNCPIS-FYWRMAATSA-N
• SMILES: C1=CC=C(C(=C1)C(=C(N)SC2=CC=C(C=C2)N)C#N)C(F)(F)F

SL-327 (CAT: I003073) is a cell-permeable vinylogous cyanamide compound that functions as a selective inhibitor of mitogen-activated protein kinase kinase 1 and 2 (MEK-1 and MEK-2). MEK is an upstream kinase in the MAPK/ERK signaling pathway and plays a crucial role in transmitting signals from cell surface receptors to the nucleus, regulating cell growth, differentiation, and survival. By inhibiting MEK-1 and MEK-2, SL-327 disrupts the activation of ERK and downstream signaling events. This selective inhibition of MEK-1 and MEK-2 by SL-327 has been widely used in research to study the MAPK/ERK pathway and explore its role in various cellular processes and diseases.

Reference

1:Combination of SL327 and Sunitinib Malate leads to an additive anti-cancer effect in doxorubicin resistant thyroid carcinoma cells. Wang W, Zhou J, Zhao L, Chen S.Biomed Pharmacother. 2017 Apr;88:985-990. doi: 10.1016/j.biopha.2017.01.135. Epub 2017 Feb 6. PMID: 28178630 2:Differential effects of the MEK inhibitor SL327 on the acquisition and expression of ethanol-elicited conditioned place preference and aversion in mice. Rosas M, Porru S, Longoni R, Spina L, Peana AT, Collu M, Acquas E.J Psychopharmacol. 2017 Jan;31(1):105-114. doi: 10.1177/0269881116675514. Epub 2016 Nov 15. PMID: 28072036 3:The MEK inhibitor SL327 blocks acquisition but not expression of lithium-induced conditioned place aversion: a behavioral and immunohistochemical study. Longoni R, Spina L, Vinci S, Acquas E.Psychopharmacology (Berl). 2011 Jul;216(1):63-73. doi: 10.1007/s00213-011-2192-9. Epub 2011 Feb 11. PMID: 21312031 4:Inhibition of extracellular signal-regulated kinase (ERK) activity with SL327 does not prevent acquisition, expression, and extinction of ethanol-seeking behavior in mice. Groblewski PA, Franken FH, Cunningham CL.Behav Brain Res. 2011 Mar 1;217(2):399-407. doi: 10.1016/j.bbr.2010.11.018. Epub 2010 Nov 11. PMID: 21074569 Free PMC Article