Z-Gly-Pro-Arg-AMC hydrochloride

• CAT Number: I040415
• CAS Number: 201928-42-9
• Molecular Formula: C31H38ClN7O7
• Molecular Weight: 656.13
• Purity: ≥95%
• Synonyms: benzyl N-[2-[(2S)-2-[[(2S)-5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate;hydrochloride
• InChI: InChI=1S/C31H37N7O7.ClH/c1-19-15-27(40)45-25-16-21(11-12-22(19)25)36-28(41)23(9-5-13-34-30(32)33)37-29(42)24-10-6-14-38(24)26(39)17-35-31(43)44-18-20-7-3-2-4-8-20;/h2-4,7-8,11-12,15-16,23-24H,5-6,9-10,13-14,17-18H2,1H3,(H,35,43)(H,36,41)(H,37,42)(H4,32,33,34);1H/t23-,24-;/m0./s1
• InChIKey: WIIZWHSOXXZHRC-UKOKCHKQSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)C3CCCN3C(=O)CNC(=O)OCC4=CC=CC=C4.Cl

Z-Gly-Pro-Arg-AMC hydrochloride(CAT: I040415) is a fluorogenic substrate specifically designed for the enzymatic activity assays of trypsin and cathepsin K. It consists of a tripeptide sequence (Z-Gly-Pro-Arg) linked to 7-amino-4-methylcoumarin (AMC), a fluorescent reporter. Upon enzymatic cleavage by trypsin or cathepsin K, AMC is released, producing a measurable fluorescent signal that correlates with protease activity. This compound is ideal for kinetic studies, inhibitor screening, and high-throughput assays. Z-Gly-Pro-Arg-AMC hydrochloride is widely used in enzymology, drug discovery, and research related to bone remodeling, inflammation, and cancer where cathepsin K and trypsin play critical physiological and pathological roles.