WEE1-IN-3

• CAT Number: I029900
• CAS Number: 2272976-28-8
• Molecular Formula: C28H31N7O2
• Molecular Weight: 497.59
• Purity: ≥95%
• Synonyms: 1-[6-(2-hydroxypropan-2-yl)pyridin-2-yl]-6-[(2-methylspiro[1,3-dihydroisoquinoline-4,1′-cyclopropane]-7-yl)amino]-2-prop-2-enylpyrazolo[3,4-d]pyrimidin-3-one
• IUPAC Name: 1-[6-(2-hydroxypropan-2-yl)pyridin-2-yl]-6-[(2-methylspiro[1,3-dihydroisoquinoline-4,1'-cyclopropane]-7-yl)amino]-2-prop-2-enylpyrazolo[3,4-d]pyrimidin-3-one
• InChI: InChI=1S/C28H31N7O2/c1-5-13-34-25(36)20-15-29-26(32-24(20)35(34)23-8-6-7-22(31-23)27(2,3)37)30-19-9-10-21-18(14-19)16-33(4)17-28(21)11-12-28/h5-10,14-15,37H,1,11-13,16-17H2,2-4H3,(H,29,30,32)
• InChIKey: JSZFIXAMFNNRKS-UHFFFAOYSA-N
• SMILES: CC(C)(C1=NC(=CC=C1)N2C3=NC(=NC=C3C(=O)N2CC=C)NC4=CC5=C(C=C4)C6(CC6)CN(C5)C)O

WEE1‑IN‑3（Cat No.:I029900）is a potent, selective small‑molecule inhibitor of the serine/threonine kinase Wee1, a key regulator of the G₂/M checkpoint and promoter of DNA damage repair. It inhibits Wee1 with an IC₅₀ below 10 nM, causing loss of inhibitory phosphorylation on CDK1 and forcing premature mitotic entry. In vitro, it suppresses proliferation of cancer cell lines, notably SW480 (100–1,000 nM IC₅₀) and NCI‑H23 (~42 nM) . By disrupting cell cycle control, WEE1‑IN‑3 induces mitotic catastrophe and apoptosis, making it a valuable tool for studying checkpoint dependency in p53‑ or RAS‑mutant cancers.