Tris Buffered Saline

• CAT Number: I041385
• Purity: ≥95%

Tris Buffered Saline (Cat No.: I041385) is a commonly used buffer solution composed of Tris (tris(hydroxymethyl)aminomethane) and sodium chloride, typically adjusted to pH 7.4. It maintains stable pH and ionic strength, making it ideal for biochemical applications such as immunohistochemistry, Western blotting, and ELISA. TBS effectively preserves protein structure and function during washing and dilution steps. Its isotonic and non-toxic nature supports consistent experimental conditions, making it a staple in molecular biology and protein analysis workflows.

Reference

[1]. Holgate CS, et al. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem. 1983 Jul;31(7):938-44.
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[2]. Wood SJ, et al. alpha-synuclein fibrillogenesis is nucleation-dependent. Implications for the pathogenesis of Parkinson’s disease. J Biol Chem. 1999 Jul 9;274(28):19509-12.
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[3]. Pagès G, et al. Mitogen-activated protein kinases p42mapk and p44mapk are required for fibroblast proliferation. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8319-23.
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[4]. Knight M, et al. Shedding of syncytiotrophoblast microvilli into the maternal circulation in pre-eclamptic pregnancies. Br J Obstet Gynaecol. 1998 Jun;105(6):632-40.
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[5]. Maniotis AJ, et al. Vascular channel formation by human melanoma cells in vivo and in vitro: vasculogenic mimicry. Am J Pathol. 1999 Sep;155(3):739-52.
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