Reference | 1. Cancer Lett. 2013 Aug 19;336(2):347-58. doi: 10.1016/j.canlet.2013.03.025. Epub
2013 Mar 31.
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Guggulsterone sensitizes glioblastoma cells to Sonic hedgehog inhibitor SANT-1
induced apoptosis in a Ras/NFκB dependent manner.
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Dixit D(1), Ghildiyal R, Anto NP, Ghosh S, Sharma V, Sen E.
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Author information: <br>
(1)National Brain Research Centre, Manesar, Haryana 122 050, India.
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Since Shh pathway effector, Gli1, is overexpressed in gliomas, we investigated
the effect of novel Shh inhibitor SANT-1 on glioma cell viability. Though SANT-1
failed to induce apoptosis, it reduced proliferation of glioma stem-like cells.
Apart from canonical Shh cascade, Gli1 is also induced by non-canonical pathways
including NFκB. Therefore, a combinatorial strategy with Ras/NFκB inhibitor,
Guggulsterone, was employed to enhance effectiveness of SANT-1. Guggulsterone
inhibited Ras and NFκB activity and sensitized cells to SANT-1 induced apoptosis
via intrinsic apoptotic mechanism. Inhibition of either Ras or NFκB activity was
sufficient to sensitize cells to SANT-1. Guggulsterone induced ERK activation
also contributed to Caspase-9 activation. Since SANT-1 and Guggulsterone
differentially target stem-like and non-stem glioma cells respectively, this
combination warrants investigation as an effective anti-glioma therapy.
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2. Biochim Biophys Acta. 2005 Feb 14;1727(2):81-6. Epub 2005 Jan 13.
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The Terminal acidic SANT 1 (Tacs1) gene of maize is expressed in tissues
containing meristems and encodes an acidic SANT domain similar to some
chromatin-remodeling complex proteins.
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Marian CO(1), Bass HW.
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Author information: <br>
(1)Department of Biological Science, Florida State University, Tallahassee, FL
32306-4370, USA.
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While screening for plant homologs of telomeric-complex proteins, we isolated a
cDNA for the Terminal acidic SANT 1 (Tacs1) gene of maize, encoding a 45-kDa
protein with a C-terminal Myb/SANT-like domain. Gene expression and protein
modeling data indicate that the TACS1 protein may function in chromatin
remodeling within shoot primordia or other meristem-containing tissues.
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