Resorufin-d6

• CAT Number: I045502
• CAS Number: 1196157-65-9
• Molecular Formula: C12HD6NO3
• Molecular Weight: 219.23
• Purity: ≥95%
• Synonyms: 1,2,4,6,8,9-hexadeuterio-7-hydroxyphenoxazin-3-one
• InChI: InChI=1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H/i1D,2D,3D,4D,5D,6D
• InChIKey: HSSLDCABUXLXKM-MZWXYZOWSA-N
• SMILES: C1=CC2=C(C=C1O)OC3=CC(=O)C=CC3=N2

Resorufin-d6 (Cat No.: I045502) is a deuterium-labeled analog of resorufin, a highly fluorescent dye commonly used in enzyme assays and metabolic studies. The incorporation of six deuterium atoms (d₆) enhances its stability and facilitates its use as an internal standard in mass spectrometry–based quantification. Resorufin-d6 retains the parent compound’s strong red fluorescence, making it suitable for kinetic measurements, enzyme activity assays (e.g., cytochrome P450 or phosphatase assays), and high-throughput screening. Its isotopic labeling enables precise analytical calibration in biochemical, pharmacological, and environmental research applications.

Reference

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216.
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[2]. Odyniec ML , et al. ‘AND’-based fluorescence scaffold for the detection of ROS/RNS and a second analyte. Chem Commun (Camb). 2018 Jul 26;54(61):8466-8469.
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