Phorbol 12-myristate 13-acetate

• CAT Number: I002165
• CAS Number: 16561-29-8
• Molecular Formula: C36H56O8
• Molecular Weight: 616.8
• Purity: ≥95%
• Target: 11.7 nM (PKC)[1]
• Solubility: 10 mM in DMSO
• Storage: -20°C
• InChI: InChI=1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1
• InChIKey: PHEDXBVPIONUQT-RGYGYFBISA-N
• SMILES: O=C(CCCCCCCCCCCCC)O[C@H]1[C@](C2(C)C)(OC(C)=O)[C@]2([H])[C@@](C=C(CO)C[C@@]3(O)[C@@]4([H])C=C(C)C3=O)([H])[C@]4(O)[C@@H]1C

Phorbol 12-myristate 13-acetate (Cat.No:I002165) is a potent tumor-promoting agent derived from the resin of the Croton tiglium plant. It activates protein kinase C (PKC), leading to a variety of cellular responses. Phorbol 12-myristate 13-acetate is widely used in research to study PKC signaling, cell proliferation, differentiation, and apoptosis in various biological systems.

Reference

1:Phorbol 12-myristate-13-acetate mediated stabilization of Leukemia Inhibitory Factor ( lif ) mRNA: Involvement of Nucleolin and PCBP1. Chakraborty A, Mukherjee S, Saha S, De S, Sengupta Bandyopadhyay S.Biochem J. 2017 May 16. pii: BCJ20170051. doi: 10.1042/BCJ20170051. [Epub ahead of print] PMID: 28512205 2:Erratum: Evaluation of effects of various drugs on platelet functions using phorbol 12-myristate 13-acetate-induced megakaryocytic human erythroid leukemia cells [Corrigendum]. [No authors listed]Drug Des Devel Ther. 2017 Feb 8;11:371. doi: 10.2147/DDDT.S132868. eCollection 2017. PMID: 28223780 Free PMC Article3:Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment. Zhou ZL, Ma J, Tong MH, Chan BP, Wong AS, Ngan AH.Int J Nanomedicine. 2016 Dec 7;11:6533-6545. eCollection 2016. PMID: 27994457 Free PMC Article4:Differential roles of PKC isoforms (PKCs) and Ca2+ in GnRH and phorbol 12-myristate 13-acetate (PMA) stimulation of p38MAPK phosphorylation in immortalized gonadotrope cells. Mugami S, Kravchook S, Rahamim-Ben Navi L, Seger R, Naor Z.Mol Cell Endocrinol. 2017 Jan 5;439:141-154. doi: 10.1016/j.mce.2016.10.031. Epub 2016 Nov 1. PMID: 27810601 5:Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells. Yang DW, Kang OH, Lee YS, Han SH, Lee SW, Cha SW, Seo YS, Mun SH, Gong R, Shin DW, Kwon DY.Int J Mol Med. 2016 Dec;38(6):1864-1870. doi: 10.3892/ijmm.2016.2781. Epub 2016 Oct 18. PMID: 27779653 6:Dietary flavones counteract phorbol 12-myristate 13-acetate-induced SREBP-2 processing in hepatic cells. Tan YQ, Wong TY, Lin SM, Leung LK.Mol Cell Biochem. 2017 Jan;424(1-2):163-172. doi: 10.1007/s11010-016-2851-6. Epub 2016 Oct 24. PMID: 27778136 7:Evaluation of effects of various drugs on platelet functions using phorbol 12-myristate 13-acetate-induced megakaryocytic human erythroid leukemia cells. Tada T, Aki K, Oboshi W, Kawazoe K, Yasui T, Hosoi E.Drug Des Devel Ther. 2016 Sep 26;10:3099-3107. eCollection 2016. Erratum in: Drug Des Devel Ther. 2017 Feb 08;11:371. PMID: 27713620 Free PMC Article8:Phorbol-12-myristate-13-acetate (PMA) mediated transcriptional regulation of Oncostatin-M. Mukherjee S, Sengupta Bandyopadhyay S.Cytokine. 2016 Dec;88:209-213. doi: 10.1016/j.cyto.2016.09.006. Epub 2016 Sep 24. PMID: 27676154 9:Phorbol-12-myristate-13-acetate induces nociceptin in human Mono Mac 6 cells via multiple transduction signalling pathways. Zhang L, Stuber F, Lippuner C, Schiff M, Stamer UM.Br J Anaesth. 2016 Aug;117(2):250-7. doi: 10.1093/bja/aew063. Epub 2016 Jun 14. PMID: 27307289 10:Phorbol 12-myristate 13-acetate promotes nuclear translocation of hepatic steroid response element binding protein-2. Wong TY, Tan YQ, Lin SM, Leung LK.Int J Biochem Cell Biol. 2016 Jun;75:1-10. doi: 10.1016/j.biocel.2016.03.010. Epub 2016 Mar 23. PMID: 27032751