PFM01(CAT: I008341) is a nuclease-specific MRE11 inhibitor. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. MRE11 within the MRE11-RAD50-NBS1 (MRN) complex acts in DNA double-strand break repair (DSBR), detection, and signaling.
| Catalog Number | I008341 |
| CAS Number | 1558598-41-6 |
| Synonyms | PFM01; PFM-01; PFM 01.;(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone |
| Molecular Formula | C14H15NO2S2 |
| Purity | 95% |
| Solubility | Soluble in DMSO |
| Storage | 0 - 4 °C for short term, or -20 °C for long term |
| IUPAC Name | (5Z)-5-[(4-hydroxyphenyl)methylidene]-3-(2-methylpropyl)-2-sulfanylidene-1,3-thiazolidin-4-one |
| InChI | InChI=1S/C14H15NO2S2/c1-9(2)8-15-13(17)12(19-14(15)18)7-10-3-5-11(16)6-4-10/h3-7,9,16H,8H2,1-2H3/b12-7- |
| InChIKey | GPURHDUTZUYAFI-GHXNOFRVSA-N |
| SMILES | CC(C)CN1C(=O)C(=CC2=CC=C(C=C2)O)SC1=S |
| Reference | </br>1: Shibata A, Moiani D, Arvai AS, Perry J, Harding SM, Genois MM, Maity R, van Rossum-Fikkert S, Kertokalio A, Romoli F, Ismail A, Ismalaj E, Petricci E, Neale MJ, Bristow RG, Masson JY, Wyman C, Jeggo PA, Tainer JA. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Mol Cell. 2014 Jan 9;53(1):7-18. doi: 10.1016/j.molcel.2013.11.003. Erratum in: Mol Cell. 2014 Jan 23;53(2):361. PubMed PMID: 24316220; PubMed Central PMCID: PMC3909494. |
