PDK1-IN-RS2

• CAT Number: I017230
• CAS Number: 1643958-89-7
• Molecular Formula: C15H9ClN2O2S3
• Molecular Weight: 380.89
• Purity: ≥95%
• Synonyms: N-(6-chloro-1,3-benzothiazol-2-yl)-1-benzothiophene-2-sulfonamide
• InChI: InChI=1S/C15H9ClN2O2S3/c16-10-5-6-11-13(8-10)22-15(17-11)18-23(19,20)14-7-9-3-1-2-4-12(9)21-14/h1-8H,(H,17,18)
• InChIKey: MZAVPBQCWWIYEQ-UHFFFAOYSA-N
• SMILES: C1=CC=C2C(=C1)C=C(S2)S(=O)(=O)NC3=NC4=C(S3)C=C(C=C4)Cl

PDK1-IN-RS2(CAT: I017230) is a substrate-selective PDK1 inhibitor that mimics the peptide docking motif (PIFtide), with a binding affinity (Kd) of 9 μM. Unlike general PDK1 inhibitors, it suppresses the activation of downstream kinases such as S6K1, demonstrating selectivity for substrate-specific regulation. Interestingly, PDK1-IN-RS2 enhances PDK1 catalytic activity toward a peptide substrate by approximately sixfold, highlighting its unique modulatory mechanism. Structural studies reveal that its sulfonyl group forms a salt bridge with Arg131, facilitated by the ionized sulfonamide under crystallization conditions. These properties make PDK1-IN-RS2 a valuable tool for dissecting PDK1 signaling and substrate-specific kinase regulation in cellular pathways.