For research use only. Not for therapeutic Use.
OSMI-2 (CAT: I016836) is a potent, cell-permeable inhibitor of O-linked N-acetylglucosamine transferase (OGT), the enzyme responsible for O-GlcNAc protein modification. Treatment with OSMI-2 (20–50 μM; 4–24 h) in HCT116 cells reduces O-GlcNAc levels at early time points (<8 h), with recovery observed at 24 h due to compensatory increases in OGT abundance. OSMI-2 disrupts HCF-1 processing, promotes detained intron splicing, and slows cell proliferation without inducing apoptosis, mimicking OGT knockdown phenotypes. By selectively modulating OGT activity and O-GlcNAc cycling, OSMI-2 provides a valuable tool for studying epigenetic regulation, transcriptional control, and metabolic signaling pathways linked to O-GlcNAcylation.
| CAS Number | 2260542-60-5 |
| Synonyms | methyl 2-[[(2R)-2-(2-methoxyphenyl)-2-[(2-oxo-1H-quinolin-6-yl)sulfonylamino]acetyl]-(thiophen-2-ylmethyl)amino]acetate |
| Molecular Formula | C26H25N3O7S2 |
| Purity | ≥95% |
| IUPAC Name | methyl 2-[[(2R)-2-(2-methoxyphenyl)-2-[(2-oxo-1H-quinolin-6-yl)sulfonylamino]acetyl]-(thiophen-2-ylmethyl)amino]acetate |
| InChI | InChI=1S/C26H25N3O7S2/c1-35-22-8-4-3-7-20(22)25(26(32)29(16-24(31)36-2)15-18-6-5-13-37-18)28-38(33,34)19-10-11-21-17(14-19)9-12-23(30)27-21/h3-14,25,28H,15-16H2,1-2H3,(H,27,30)/t25-/m1/s1 |
| InChIKey | ACLDUOSWRUIOSB-RUZDIDTESA-N |
| SMILES | COC1=CC=CC=C1C(C(=O)N(CC2=CC=CS2)CC(=O)OC)NS(=O)(=O)C3=CC4=C(C=C3)NC(=O)C=C4 |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
