NS-398

• CAT Number: I000945
• CAS Number: 123653-11-2
• Molecular Formula: C13H18N2O5S
• Molecular Weight: 314.4
• Purity: ≥95%
• Synonyms: N-(2-(cyclohexyloxy)-4-nitrophenyl)methanesulfonamide
• Target: COX
• Solubility: 10 mM in DMSO
• Storage: Room Temperature
• IC50: 3.8 μM
• IUPAC Name: N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
• InChI: InChI=1S/C13H18N2O5S/c1-21(18,19)14-12-8-7-10(15(16)17)9-13(12)20-11-5-3-2-4-6-11/h7-9,11,14H,2-6H2,1H3
• InChIKey: KTDZCOWXCWUPEO-UHFFFAOYSA-N
• SMILES: CS(=O)(=O)NC1=C(C=C(C=C1)[N+](=O)[O-])OC2CCCCC2

NS-398 (Cat No.:I000945) is a selective COX-2 inhibitor with IC50 of 75 μM and 1.77 μM for human recombinant COX-1 and COX-2, respectively. NS398 can inhibit the expression of COX-2 induced by auditory damage, and relieve noise-induced hearing threshold changes and hair cell loss.

Reference

1:Novel effects of the cyclooxygenase-2-selective inhibitor NS-398 on IL-1β-induced cyclooxygenase-2 and IL-8 expression in human ovarian granulosa cells. Ou HL, Sun D, Peng YC, Wu YL.Innate Immun. 2016 Aug;22(6):452-65. doi: 10.1177/1753425916654011. Epub 2016 Jun 16. PMID: 27312705 2:NS-398 promotes pancreatic cancer cell invasion by CD147 and MMP-2 via the activation of P38. Liu H, Xu XF, Zhao Y, Tang MC, Zhou YQ, Gao FH.Mol Med Rep. 2016 Mar;13(3):2208-14. doi: 10.3892/mmr.2016.4783. Epub 2016 Jan 14. PMID: 26782265 3:The COX-2-Selective Antagonist (NS-398) Inhibits Choroidal Neovascularization and Subretinal Fibrosis. Zhang R, Liu Z, Zhang H, Zhang Y, Lin D.PLoS One. 2016 Jan 13;11(1):e0146808. doi: 10.1371/journal.pone.0146808. eCollection 2016. PMID: 26760305 Free PMC Article4:COX-2 inhibitor NS-398 suppresses doxorubicin-induced p53 accumulation through inhibition of ROS-mediated Jnk activation. Kim J, Shim M.Mol Carcinog. 2016 Dec;55(12):2156-2167. doi: 10.1002/mc.22458. Epub 2016 Jan 12. PMID: 26756900 5:Retraction Note to: Effect of NS-398, a cyclooxygenase-2 selective inhibitor, on the cytotoxicity of cytotoxic T lymphocytes to ovarian carcinoma cells. [No authors listed]Tumour Biol. 2015 Sep;36(9):7297. doi: 10.1007/s13277-015-3694-6. No abstract available. PMID: 26281984 6:Co-administration of subtherapeutic diazepam enhances neuroprotective effect of COX-2 inhibitor, NS-398, after lithium pilocarpine-induced status epilepticus. Trandafir CC, Pouliot WA, Dudek FE, Ekstrand JJ.Neuroscience. 2015 Jan 22;284:601-10. doi: 10.1016/j.neuroscience.2014.10.021. Epub 2014 Oct 18. PMID: 25453777 Free PMC Article7:NS-398 enhances the efficacy of bortezomib against RPMI8226 human multiple myeloma cells. Que W, Li S, Chen J.Mol Med Rep. 2013 May;7(5):1641-5. doi: 10.3892/mmr.2013.1394. Epub 2013 Mar 26. PMID: 23545701 8:Effect of NS-398, a cyclooxygenase-2 selective inhibitor, on the cytotoxicity of cytotoxic T lymphocytes to ovarian carcinoma cells. Wang X, Liang Y, Wang J, Wang M.Tumour Biol. 2013 Jun;34(3):1517-22. doi: 10.1007/s13277-013-0677-3. Epub 2013 Mar 1. Retraction in: Wang X, Liang Y, Wang J, Wang M. Tumour Biol. 2015 Aug;36(9):7297. PMID: 23456767 9:NS-398 reverses hypotension in endotoxemic rats: contribution of eicosanoids, NO, and peroxynitrite. Tunctan B, Sari AN, Kacan M, Unsal D, Buharalioglu CK, Sahan-Firat S, Korkmaz B, Falck JR, Malik KU.Prostaglandins Other Lipid Mediat. 2013 Jul-Aug;104-105:93-108. doi: 10.1016/j.prostaglandins.2012.08.007. Epub 2012 Sep 5. PMID: 22975359 Free PMC Article10:The cyclooxygenase-2 inhibitor NS-398 inhibits proliferation and induces apoptosis in human osteosarcoma cells via downregulation of the survivin pathway. Duan DP, Dang XQ, Wang KZ, Wang YP, Zhang H, You WL.Oncol Rep. 2012 Nov;28(5):1693-700. doi: 10.3892/or.2012.1992. Epub 2012 Aug 24. PMID: 22922684