Nitrosobiotin

• CAT Number: I016848
• CAS Number: 56859-26-8
• Molecular Formula: C10H15N3O4S
• Molecular Weight: 273.31
• Purity: ≥95%
• Synonyms: 5-[(3aR,6S,6aS)-3-nitroso-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]pentanoic acid
• InChI: InChI=1S/C10H15N3O4S/c14-8(15)4-2-1-3-7-9-6(5-18-7)13(12-17)10(16)11-9/h6-7,9H,1-5H2,(H,11,16)(H,14,15)/t6-,7-,9-/m0/s1
• InChIKey: IFUXQNNMTPBRPW-ZKWXMUAHSA-N
• SMILES: C1C2C(C(S1)CCCCC(=O)O)NC(=O)N2N=O

Nitrosobiotin(CAT: I016848) is a biotin derivative functionalized with a nitroso group, making it a versatile probe for biochemical and chemical biology applications. The nitroso moiety provides a reactive handle for selective coupling, enabling covalent interactions with thiols, amines, and other nucleophilic residues. Retaining the biotin scaffold, Nitrosobiotin maintains strong binding affinity to avidin and streptavidin, allowing its use in affinity capture, labeling, and detection assays. Its dual functionality makes it particularly valuable for redox biology, protein modification studies, and the design of crosslinking reagents. Nitrosobiotin expands the utility of the biotin–streptavidin system into dynamic and covalent biochemical applications.