NICE-01

• CAT Number: I040568
• CAS Number: 2982819-94-1
• Molecular Formula: C63H76ClN7O13S
• Molecular Weight: 1206.83
• Purity: ≥95%
• Synonyms: [(1R)-1-[3-[2-[2-[2-[2-[[2-[(9S)-7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.02,6]trideca-2(6),4,7,10,12-pentaen-9-yl]acetyl]amino]ethoxy]ethoxy]ethylamino]-2-oxoethoxy]phenyl]-3-(3,4-dimethoxyphenyl)propyl] (2S)-1-[(2S)-2-(3,4,5-trimethoxyphenyl)butanoyl]piperidine-2-carboxylate
• InChI: InChI=1S/C63H76ClN7O13S/c1-10-47(44-34-53(78-7)59(80-9)54(35-44)79-8)61(74)70-27-12-11-16-49(70)63(75)84-50(23-17-41-18-24-51(76-5)52(32-41)77-6)43-14-13-15-46(33-43)83-37-56(73)66-26-29-82-31-30-81-28-25-65-55(72)36-48-60-69-68-40(4)71(60)62-57(38(2)39(3)85-62)58(67-48)42-19-21-45(64)22-20-42/h13-15,18-22,24,32-35,47-50H,10-12,16-17,23,25-31,36-37H2,1-9H3,(H,65,72)(H,66,73)/t47-,48-,49-,50+/m0/s1
• InChIKey: USCBOLCYUGPYAF-YUZLDYPQSA-N
• SMILES: CC[C@@H](C1=CC(=C(C(=C1)OC)OC)OC)C(=O)N2CCCC[C@H]2C(=O)O[C@H](CCC3=CC(=C(C=C3)OC)OC)C4=CC(=CC=C4)OCC(=O)NCCOCCOCCNC(=O)C[C@H]5C6=NN=C(N6C7=C(C(=C(S7)C)C)C(=N5)C8=CC=C(C=C8)Cl)C

NICE-01(CAT: I040568) (also known as AP1867-PEG2-JQ1 or AP-PEG2-JQ1) is a bifunctional chemical tool designed to mediate the nuclear import of cytosolic proteins. It simultaneously binds cytosolic FKBP-tagged cargo proteins and the nuclear-localized bromodomain-containing protein BRD4, functioning as a molecular bridge. By leveraging BRD4 as a “carrier,” NICE-01 facilitates the co-import and stable nuclear trapping of target proteins that are otherwise cytosolic. This compound offers a powerful approach for studying spatial regulation of protein function, controlled nuclear translocation, and synthetic manipulation of subcellular localization, with potential applications in gene regulation research, synthetic biology, and targeted delivery systems.