For research use only. Not for therapeutic Use.
ML334 (CAT: I011257) is a potent, cell-permeable activator of NRF2 that functions by inhibiting the Keap1–NRF2 protein-protein interaction. It binds to the Keap1 Kelch domain with a Kd of 1 μM, preventing NRF2 degradation and promoting its stabilization and nuclear translocation. ML334 subsequently enhances antioxidant response element (ARE) activity, leading to upregulation of cytoprotective genes. In HEK293 cells, treatment with ML334 (50–100 μM) increases NQO1 and TRX1 mRNA 2–3 fold and HO-1 mRNA up to 7 fold, while also inducing HO-1 and TRX1 proteins. ML334 is a valuable research probe for redox biology and oxidative stress–related pathways.
| CAS Number | 1432500-66-7 |
| Synonyms | (1S,2R)-2-[(1S)-1-[(1,3-dioxoisoindol-2-yl)methyl]-3,4-dihydro-1H-isoquinoline-2-carbonyl]cyclohexane-1-carboxylic acid |
| Molecular Formula | C26H26N2O5 |
| Purity | ≥95% |
| IUPAC Name | (1S,2R)-2-[(1S)-1-[(1,3-dioxoisoindol-2-yl)methyl]-3,4-dihydro-1H-isoquinoline-2-carbonyl]cyclohexane-1-carboxylic acid |
| InChI | InChI=1S/C26H26N2O5/c29-23(20-11-5-6-12-21(20)26(32)33)27-14-13-16-7-1-2-8-17(16)22(27)15-28-24(30)18-9-3-4-10-19(18)25(28)31/h1-4,7-10,20-22H,5-6,11-15H2,(H,32,33)/t20-,21+,22-/m1/s1 |
| InChIKey | LNENLABLFGGAFF-BHIFYINESA-N |
| SMILES | C1CCC(C(C1)C(=O)N2CCC3=CC=CC=C3C2CN4C(=O)C5=CC=CC=C5C4=O)C(=O)O |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
