MIF-Antagonist, ISO-1

• CAT Number: I003675
• CAS Number: 478336-92-4
• Molecular Formula: C₁₂H₁₃NO₄
• Molecular Weight: 235.24
• Purity: ≥95%
• Target: MIF inhibitor
• Solubility: DMSO: ≥ 36 mg/mL
• Storage: -20°C
• IC50: 7 uM [1]
• InChI: InChI=1S/C12H13NO4/c1-16-12(15)7-10-6-11(13-17-10)8-2-4-9(14)5-3-8/h2-5,10,13H,6-7H2,1H3
• InChIKey: CLVQGFPQFNASJH-UHFFFAOYSA-N
• SMILES: COC(=O)CC1CC(=C2C=CC(=O)C=C2)NO1

ISO-1(CAT: I003675) operates as an antagonist of macrophage migration inhibitory factor (MIF). This compound specifically targets MIF, an important regulatory protein. In vitro, studies demonstrate that ISO-1 hinders MIF tautomerase activity in a dose-dependent manner, with the hydroxylated phenyl analog being less potent. ISO-1’s potential mechanism includes the modulation of arachidonic acid release, while its application curtails cytokine activity and tumor necrosis factor release from macrophages. In vivo, ISO-1 administration showcases promising outcomes, leading to reduced implant size and vascularity, potentially influencing therapeutic strategies involving MIF-related processes and responses.

Reference

[1]. Lubetsky JB, et al. The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J Biol Chem. 2002 Jul 12;277(28):24976-82.
[2]. Al-Abed Y, et al. ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J Biol Chem. 2005 Nov 4;280(44):36541-4.
[3]. Nothnick WB, et al. Inhibition of macrophage migration inhibitory factor reduces endometriotic implant size in mice with experimentally induced disease. J Endometr. 2011 Sep 30;3(3):135-142.