Hoechst 33342

• CAT Number: I002780
• CAS Number: 23491-52-3
• Molecular Formula: C27H28N6O
• Molecular Weight: 452.55
• Purity: ≥95%
• Target: DNA Stain
• Solubility: DMSO or water Protect from light
• Storage: Store at -20°C
• InChI: InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H
• InChIKey: JABNPSKWVNCGMX-UHFFFAOYSA-N
• SMILES: CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1.[H]Cl.[H]Cl.[H]Cl

Hoechst 33342 (CAT: I002780) is a fluorescent dye and a cell-permeable DNA stain commonly used in molecular biology and cell biology research. It binds to the minor groove of double-stranded DNA, exhibiting preferential binding to AT-rich regions. Hoechst 33342 is widely used for nuclear staining and DNA content analysis in live or fixed cells. It is also employed in fluorescence microscopy and flow cytometry to visualize and quantify DNA, identify cell cycle phases, and assess cell viability. Furthermore, Hoechst 33342 has been utilized in studies involving DNA damage, apoptosis, and cell sorting based on DNA content.

Reference

1:Requirement of ABC transporter inhibition and Hoechst 33342 dye deprivation for the assessment of side population-defined C6 glioma stem cell metabolism using fluorescent probes. Murota Y, Tabu K, Taga T.BMC Cancer. 2016 Nov 4;16(1):847. PMID: 27814696 Free PMC Article2:Analyzing Cell Death by Nuclear Staining with Hoechst 33342. Crowley LC, Marfell BJ, Waterhouse NJ.Cold Spring Harb Protoc. 2016 Sep 1;2016(9):pdb.prot087205. doi: 10.1101/pdb.prot087205. PMID: 27587774 3:Cell Cycle Analysis of CML Stem Cells Using Hoechst 33342 and Propidium Iodide. DeSouza N, Zhou M, Shan Y.Methods Mol Biol. 2016;1465:47-57. doi: 10.1007/978-1-4939-4011-0_5. PMID: 27581138 4:Toxicity of Hoechst 33342: implication in side population analysis. Zheng J, Qi S, Zhu H, Xiao X.Cell Mol Biol (Noisy-le-grand). 2016 Jun 30;62(7):27-30. PMID: 27453268 5:Labeling Adipose-Derived Stem Cells with Hoechst 33342: Usability and Effects on Differentiation Potential and DNA Damage. Schendzielorz P, Froelich K, Rak K, Gehrke T, Scherzad A, Hagen R, Radeloff A.Stem Cells Int. 2016;2016:6549347. doi: 10.1155/2016/6549347. Epub 2016 Jun 8. PMID: 27375746 Free PMC Article6:Hoechst 33342 Staining Identifies the Progenitor Side Population in NOD.Cg-PrkdcscidIL2rgtmWjl/Sz Mice Harboring Pediatric Leukemias. Andre MC, Witte KE, Hoerrmann RA, Schmitt A, Handgretinger R, Ebinger M.In Vivo. 2015 Nov-Dec;29(6):661-9. PMID: 26546522 7:Hoechst 33342 Is a Hidden /Janus/ amongst Substrates for the Multidrug Efflux Pump LmrP. Neuberger A, van Veen HW.PLoS One. 2015 Nov 5;10(11):e0141991. doi: 10.1371/journal.pone.0141991. eCollection 2015. PMID: 26540112 Free PMC Article8:The effects of hoechst 33342 staining and the male sample donor on the sorting efficiency of canine spermatozoa. Rodenas C, Lucas X, Tarantini T, Del Olmo D, Roca J, Vazquez JM, Martinez EA, Parrilla I.Reprod Domest Anim. 2014 Feb;49(1):115-21. doi: 10.1111/rda.12238. Epub 2013 Oct 1. PMID: 24118597 9:Revealing spectral features in two-photon absorption spectrum of Hoechst 33342: a combined experimental and quantum-chemical study. Olesiak-Banska J, Matczyszyn K, Zaleśny R, Murugan NA, Kongsted J, Ågren H, Bartkowiak W, Samoc M.J Phys Chem B. 2013 Oct 10;117(40):12013-9. doi: 10.1021/jp407144k. Epub 2013 Sep 25. PMID: 24016295 10:Evaluation of a side population of canine lymphoma cells using Hoechst 33342 dye. Kim MC, D/’Costa S, Suter S, Kim Y.J Vet Sci. 2013;14(4):481-6. Epub 2013 Jun 30. PMID: 23820219 Free PMC Article