EC 3.5.1.11

• CAT Number: M017563
• CAS Number: 9014-06-6
• Purity: ≥95%
• Storage: 2-8°C

EC 3.5.1.11 (Cat.No:M017563), also known as Penicillin amidase,  is the enzyme used commercially for the production of semisynthetic penicillins. It was used to study its effect in release of fatty acid and HSL (homoserine lactone) from AHLs (N-acylhomoserine lactones) in degradation of antibiotics. It was used as positive control for assaying penicillin G acylase activity in the study of functional analysis of bile salt hydrolase and penicillin acylase family members in Lactobacillus sp. Penicillin amidase may be used for synthesis of 6-aminopenicillanic acid from penicillin-G and for the industrial production of β-lactam antibiotics.

Reference

1.Sakaguchi, K. and Murao, S.A preliminary report on a new enzyme, "penicillin-amidase".

J. Agric. Chem. Soc. Jpn. 23: 411 (1950).
2.Mali, R. S., V. K. Sudhakaran, and J. G. Shewale. "Affinity and hydrophobic interactions of penicillin amidase." Hindustan Antibiotics Bulletin 31.1-2 (1989): 25-28.
3.Valle, Fernando, et al. "The role of penicillin amidases in nature and in industry." Trends in biochemical sciences 16 (1991): 36-40.
4.Lin, Yi‐Han, et al. "Acyl‐homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum‐quenching enzymes." Molecular microbiology 47.3 (2003): 849-860.
5.Lambert, Jolanda M., et al. "Functional analysis of four bile salt hydrolase and penicillin acylase family members in Lactobacillus plantarum WCFS1." Applied and environmental microbiology 74.15 (2008): 4719-4726.