Digoxigenin

• CAT Number: I002184
• CAS Number: 1672-46-4
• Molecular Formula: C23H34O5
• Molecular Weight: 390.51
• Purity: ≥95%
• Synonyms: Digoxigenin; Lanadigenin
• Solubility: 10 mM in DMSO
• Storage: 3 years -20C powder
• InChI: InChI=1S/C23H34O5/c1-21-7-5-15(24)10-14(21)3-4-17-18(21)11-19(25)22(2)16(6-8-23(17,22)27)13-9-20(26)28-12-13/h9,14-19,24-25,27H,3-8,10-12H2,1-2H3/t14-,15+,16-,17-,18+,19-,21+,22+,23+/m1/s1
• InChIKey: SHIBSTMRCDJXLN-KCZCNTNESA-N
• SMILES: CC12CCC(CC1CCC3C2CC(C4(C3(CCC4C5=CC(=O)OC5)O)C)O)O

Digoxigenin (CAT: I002184) is a small molecule derived from the plant Digitalis purpurea, commonly known as foxglove. It is widely used in molecular biology and biochemistry as a probe for the detection and localization of nucleic acids. Digoxigenin is often conjugated to nucleotides or antibodies to facilitate its binding to specific target molecules. In nucleic acid research, digoxigenin-labeled probes are used for techniques such as in situ hybridization, Southern blotting, and Northern blotting to visualize and study the presence and expression of specific genes. The binding of digoxigenin to target molecules can be detected using immunological methods, such as enzyme-linked immunosorbent assays (ELISAs) or immunohistochemistry.

Reference

1: Baumgart E, Schad A, Völkl A, Fahimi HD. Detection of mRNAs encoding
peroxisomal proteins by non-radioactive in situ hybridization with
digoxigenin-labelled cRNAs. Histochem Cell Biol. 1997 Oct-Nov;108(4-5):371-9.
Review. PubMed PMID: 9387930.

2: Chevalier J, Yi J, Michel O, Tang XM. Biotin and digoxigenin as labels for
light and electron microscopy in situ hybridization probes: where do we stand? J
Histochem Cytochem. 1997 Apr;45(4):481-91. Review. PubMed PMID: 9111227.

3: Höltke HJ, Ankenbauer W, Mühlegger K, Rein R, Sagner G, Seibl R, Walter T. The
digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic
acids–an overview. Cell Mol Biol (Noisy-le-grand). 1995 Nov;41(7):883-905.
Review. PubMed PMID: 8595368.