DiFMUP

• CAT Number: R071577
• CAS Number: 214491-43-7
• Molecular Formula: C10H7F2O6P
• Molecular Weight: 292.13
• Purity: ≥95%
• Synonyms: (6,8-difluoro-4-methyl-2-oxochromen-7-yl) dihydrogen phosphate
• InChI: InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
• InChIKey: DZANYXOTJVLAEE-UHFFFAOYSA-N
• SMILES: CC1=CC(=O)OC2=C(C(=C(C=C12)F)OP(=O)(O)O)F

DiFMUP (Cat No.: R071577) is a widely used fluorogenic substrate for phosphatase assays. Upon enzymatic dephosphorylation, it releases difluoro-4-methylumbelliferone, a highly fluorescent product, enabling sensitive detection of phosphatase activity. Its strong signal-to-noise ratio, stability, and compatibility with high-throughput screening make DiFMUP ideal for biochemical and cell-based assays. Researchers employ it to study protein tyrosine phosphatases and other phosphatase families, supporting drug discovery, enzymology, and signal transduction research by providing a robust tool for monitoring enzyme kinetics and inhibitor activity.

Reference

[1]. Gee KR, et, al. Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases. Anal Biochem. 1999 Aug 15;273(1):41-8.
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[2]. Welte S, et, al. 6,8-Difluoro-4-methylumbiliferyl phosphate: a fluorogenic substrate for protein tyrosine phosphatases. Anal Biochem. 2005 Mar 1;338(1):32-8.
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