DBCO-PEG2-NHS ester

• CAT Number: I042574
• CAS Number: 2585653-12-7
• Molecular Formula: C30H31N3O8
• Molecular Weight: 561.58
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[[4-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-4-oxobutanoyl]amino]ethoxy]ethoxy]propanoate
• InChI: InChI=1S/C30H31N3O8/c34-26(31-16-18-40-20-19-39-17-15-30(38)41-33-28(36)13-14-29(33)37)11-12-27(35)32-21-24-7-2-1-5-22(24)9-10-23-6-3-4-8-25(23)32/h1-8H,11-21H2,(H,31,34)
• InChIKey: WEMCNVHTFXFLHG-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCOCCOCCNC(=O)CCC(=O)N2CC3=CC=CC=C3C#CC4=CC=CC=C42

DBCO-PEG2-NHS ester (Cat No.: I042574) is a heterobifunctional crosslinker combining a dibenzocyclooctyne (DBCO) group and an N-hydroxysuccinimide (NHS) ester linked by a short PEG2 spacer. The NHS ester reacts with primary amines on proteins or biomolecules, while the DBCO moiety enables copper-free click chemistry with azides via strain-promoted alkyne-azide cycloaddition (SPAAC). This reagent allows efficient, site-specific bioconjugation under mild conditions. Its PEG2 linker enhances solubility and flexibility, making it ideal for labeling, drug delivery, and biomolecular engineering applications in complex biological environments.

Reference

[1]. Jiang X, et al. Recent applications of click chemistry in drug discovery. Expert Opin Drug Discov. 2019 Aug;14(8):779-789.
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