CIL56

• CAT Number: I011853
• CAS Number: 300802-28-2
• Molecular Formula: C23H27N3O5S2
• Molecular Weight: 489.6
• Purity: ≥95%
• Storage: -20°C
• InChI: InChI=1S/C23H27N3O5S2/c27-24-23-21-15-17(32(28,29)25-11-3-1-4-12-25)7-9-19(21)20-10-8-18(16-22(20)23)33(30,31)26-13-5-2-6-14-26/h7-10,15-16,27H,1-6,11-14H2
• InChIKey: XYZXEEIUKQGUHB-UHFFFAOYSA-N
• SMILES: O=S(C1=CC2=C(C=C1)C3=CC=C(S(N4CCCCC4)(=O)=O)C=C3/C2=NO)(N5CCCCC5)=O

CIL56 (CAT: I011853) is a small molecule that triggers cellular ferroptosis, a form of regulated cell death characterized by iron-dependent reactive oxygen species (ROS) production. In addition, CIL56 induces the accumulation of long-chain saturated, monounsaturated, and polyunsaturated fatty acids in HT-1080 cells in vitro. It has been observed that HT-1080 cells lacking acetyl-CoA carboxylase 1 (ACC1), the key enzyme involved in fatty acid synthesis, exhibit a five-fold resistance to CIL56-induced cell death, suggesting that ACC1 activity sensitizes cells to CIL56 treatment. These findings highlight the potential of CIL56 as a modulator of ferroptosis and fatty acid metabolism, with implications for developing novel therapeutic strategies targeting these processes.

Reference

1. ACS Chem Biol. 2015 Jul 17;10(7):1604-9. doi: 10.1021/acschembio.5b00245. Epub
2015 May 15.

Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in
Nonapoptotic Cell Death.

Dixon SJ(1), Winter GE(2), Musavi LS, Lee ED, Snijder B(2), Rebsamen M(2),
Superti-Furga G(2), Stockwell BR.

Author information:
(1)⊥Department of Biology, Stanford University, 337 Campus Drive, Stanford,
California 94305, United States.
(2)∥CeMM Research Center for Molecular Medicine of the Austrian Academy of
Sciences, Lazarettgasse 14, 1090 Vienna, Austria.

Little is known about the regulation of nonapoptotic cell death. Using massive
insertional mutagenesis of haploid KBM7 cells we identified nine genes involved
in small-molecule-induced nonapoptotic cell death, including mediators of fatty
acid metabolism (ACSL4) and lipid remodeling (LPCAT3) in ferroptosis. One novel
compound, CIL56, triggered cell death dependent upon the rate-limiting de novo
lipid synthetic enzyme ACC1. These results provide insight into the genetic
regulation of cell death and highlight the central role of lipid metabolism in
nonapoptotic cell death.