Biotin-PEG4-azide

• CAT Number: I045979
• CAS Number: 1309649-57-7
• Molecular Formula: C20H36N6O6S
• Molecular Weight: 488.60
• Purity: ≥95%
• Synonyms: 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethyl]pentanamide
• InChI: InChI=1S/C20H36N6O6S/c21-26-23-6-8-30-10-12-32-14-13-31-11-9-29-7-5-22-18(27)4-2-1-3-17-19-16(15-33-17)24-20(28)25-19/h16-17,19H,1-15H2,(H,22,27)(H2,24,25,28)/t16-,17-,19-/m0/s1
• InChIKey: PVEHVEYAPUNCCP-LNLFQRSKSA-N
• SMILES: C1C2C(C(S1)CCCCC(=O)NCCOCCOCCOCCOCCN=[N+]=[N-])NC(=O)N2

Biotin-PEG4-azide(CAT: I045979) is a biotinylated probe with a terminal azide group and a PEG4 spacer designed for efficient bioconjugation. The azide moiety enables copper-catalyzed azide–alkyne cycloaddition (CuAAC, “click chemistry”) with alkyne-containing molecules, yielding stable triazole linkages. The PEG4 chain enhances water solubility, reduces steric hindrance, and improves flexibility, ensuring efficient labeling reactions. The biotin tag allows strong, specific binding to streptavidin or avidin systems, facilitating detection, affinity purification, and immobilization. Biotin-PEG4-azide is widely applied in chemical biology, proteomics, and drug discovery for probe construction, molecular labeling, and biomolecular tracking using click chemistry.