BI-6C9

• CAT Number: R065495
• CAS Number: 791835-21-7
• Molecular Formula: C23H25N3O4S2
• Molecular Weight: 471.59
• Purity: ≥95%
• Storage: -20°C
• IUPAC Name: N-[4-(4-aminophenyl)sulfanylphenyl]-4-[(4-methoxyphenyl)sulfonylamino]butanamide
• InChI: InChI=1S/C23H25N3O4S2/c1-30-19-8-14-22(15-9-19)32(28,29)25-16-2-3-23(27)26-18-6-12-21(13-7-18)31-20-10-4-17(24)5-11-20/h4-15,25H,2-3,16,24H2,1H3,(H,26,27)
• InChIKey: LCFUJBSKPDPGKO-UHFFFAOYSA-N
• SMILES: COC1=CC=C(C=C1)S(=O)(=O)NCCCC(=O)NC2=CC=C(C=C2)SC3=CC=C(C=C3)N

BH3-interacting domain (Bid) is cleaved by caspase-8 to produce the truncated protein tBid, which facilitates cytochrome c release from mitochondria and drives apoptosis. BI-6C9 is an inhibitor of tBid (Kd = 20 µM), blocking the release of both cytochrome c and second mitochondria-derived activator of caspase from mitochondria. It prevents apoptosis in neurons treated with glutamate or oxygen-glucose deprivation. BI-6C9 (10 µM) also inhibits the nuclear translocation of apoptosis-inducing factor AIF, preventing the death of ovarian OVCAR-3 cancer cells induced by IFN-α2a.

Reference

1.Dejean, L.M.,Ryu, S.Y.,Martinez-Caballero, S., et al. MAC and Bcl-2 family proteins conspire in a deadly plot. Biochimica et Biophysica Acta 1797(6-7), 1231-1238 (2010).