B022

• CAT Number: I017424
• CAS Number: 1202764-53-1
• Molecular Formula: C19H16ClN5OS
• Molecular Weight: 397.88
• Purity: ≥95%
• Synonyms: 4-[1-(2-amino-5-chloropyrimidin-4-yl)-2,3-dihydroindol-6-yl]-2-(1,3-thiazol-2-yl)but-3-yn-2-ol
• InChI: InChI=1S/C19H16ClN5OS/c1-19(26,17-22-7-9-27-17)6-4-12-2-3-13-5-8-25(15(13)10-12)16-14(20)11-23-18(21)24-16/h2-3,7,9-11,26H,5,8H2,1H3,(H2,21,23,24)
• InChIKey: OKFYOOFXVBCIIP-UHFFFAOYSA-N
• SMILES: CC(C#CC1=CC2=C(CCN2C3=NC(=NC=C3Cl)N)C=C1)(C4=NC=CS4)O

B022(CAT: I017424) is a potent and selective NF-κB-inducing kinase (NIK) inhibitor with a Kᵢ of 4.2 nM and IC₅₀ of 15.1 nM. By blocking NIK activity, B022 suppresses p52 formation and inhibits the expression of pro-inflammatory mediators including TNF-α, IL-6, iNOS, CCL2, and CXCL5 in Hepa1 cells. In vivo, it protects against toxin- and NIK-induced liver inflammation, oxidative stress, and injury, and prevents β-cell death in diabetes models, thereby reducing hyperglycemia. Additionally, B022 contains an alkyne functional group, enabling copper-catalyzed azide–alkyne cycloaddition (CuAAC) for chemical biology applications. This dual utility makes B022 valuable in inflammation, metabolic disease, and chemical probe research.