Amiloride HCl

• CAT Number: A000184
• CAS Number: 2016-88-8
• Molecular Formula: C6H8ClN7O • HCl
• Molecular Weight: 266.1
• Purity: ≥95%
• Target: Sodium channel
• Storage: 3 years -20C powder
• InChIKey: ACHKKGDWZVCSNH-UHFFFAOYSA-N

It is an epithelial channel blocker, it also blocks voltage-gated Na+ channels. It has an anti-hypertensive action.Pyrazinoylguanidine<!–C<SUB>6</SUB>H<SUB>8</SUB>ClN<SUB>7</SUB>O .HCl–>, syntheticIt is a potassium-sparing diuretic, ion transporters, enzymes and receptors inhibitor. 10-6 M reversibly inhibit Na+ transport in Na+ channels of the high resistance apical plasma epithelial membrane, when added to the solution bathing this membrane. It has no effect on Na+ transport in basolateral plasma membrane [3-4]. The Na+ channel is the only ion transporter inhibited by Amiloride with an IC50 of less than 1 μM. IC50 in urinary epithelia = 0.34 μM, evaluated by the method of Cuthbert et al. 1978 [1]. it is a weak reversible inhibitor of Na+/Ca2+ exchanger. IC50 = 2 mM for Na+-coupled solute transport in brush border membrane vesicles from rabbit kidney cortex and in LLC-PK1/CL4 cells [5].

Reference

– Kleyman T.R. et al., /Amiloride and its analogs as tools in the study of ion transport./ J. Membr. Biol. (1988). 105:1-21- Garritsen A. et al., /Interaction of Amiloride and its analogs with Adenosine A1 receptors in calf bain/, Biochem Pharmacol. (1990). 40(4):827- Bently P.J., J. Physiol. (London) (1968). 195:317-330- Sariban-Sohraby S. et al., Am. J. Physiol. (1986). 250:C175-C190- Harris R.C. et al., Kidney Int. (1985). 27:310