Ac-Nle-Pro-Nle-Asp-AMC

• CAT Number: R067000
• CAS Number: 355140-49-7
• Molecular Formula: C33H45N5O9
• Molecular Weight: 655.74
• Purity: ≥95%
• Synonyms: (3S)-3-[[(2S)-2-[[(2S)-1-[(2S)-2-acetamidohexanoyl]pyrrolidine-2-carbonyl]amino]hexanoyl]amino]-4-[(4-methyl-2-oxochromen-7-yl)amino]-4-oxobutanoic acid
• InChI: InChI=1S/C33H45N5O9/c1-5-7-10-23(36-32(45)26-12-9-15-38(26)33(46)24(11-8-6-2)34-20(4)39)30(43)37-25(18-28(40)41)31(44)35-21-13-14-22-19(3)16-29(42)47-27(22)17-21/h13-14,16-17,23-26H,5-12,15,18H2,1-4H3,(H,34,39)(H,35,44)(H,36,45)(H,37,43)(H,40,41)/t23-,24-,25-,26-/m0/s1
• InChIKey: WPTAROWBMZVECB-CQJMVLFOSA-N
• SMILES: CCCC[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C)NC(=O)[C@@H]3CCCN3C(=O)[C@H](CCCC)NC(=O)C

Ac-Nle-Pro-Nle-Asp-AMC（Cat No.:R067000）is a fluorogenic peptide substrate commonly used to measure the enzymatic activity of caspases, particularly caspase-3 and related proteases involved in apoptosis. The peptide sequence contains Nle (norleucine) and is capped with AMC (7-amino-4-methylcoumarin), a fluorophore that emits fluorescence upon cleavage at the aspartic acid residue. This allows real-time monitoring of protease activity in cell lysates or biochemical assays. The acetyl (Ac) group at the N-terminus enhances stability. Ac-Nle-Pro-Nle-Asp-AMC is widely employed in apoptosis research, drug screening, and studies of programmed cell death mechanisms.