Ac-IETD-AFC

• CAT Number: R065567
• CAS Number: 211990-57-7
• Molecular Formula: C31H38F3N5O12
• Molecular Weight: 729.7
• Purity: ≥95%
• Synonyms: N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-Trifluoromethylcoumarin
• Storage: -20°C
• InChI: InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49
• InChIKey: CGUOWXRXLQXOKO-BYLHTXFJSA-N
• SMILES: OC(C[C@H](NC(C(NC([C@@H](NC([C@H]([C@@H](C)CC)NC(C)=O)=O)CCC(O)=O)=O)[C@@H](C)O)=O)C(NC(C=C1O2)=CC=C1C(C(F)(F)F)=CC2=O)=O)=O

Ac-IETD-AFC is a fluorogenic substrate that can be cleaved by caspase-8 and related enzymes that recognize the amino acid sequence IETD, including caspase-3 processing enzyme, caspase-10, and granzyme B. Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

Reference

1.Reszka, P.,Schulz, R.,Methling, K., et al. Synthesis, enzymatic evaluation, and docking studies of fluorogenic caspase 8 tetrapeptide substrates. ChemMedChem 5, 103-117 (2010).