Recombinant Human Programmed Cell Death Protein 1 (PDCD1) Protein (His-SUMO)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q15116
• Gene: PDCD1
• CAT.NO: B000212
• Expression Systems: E.coli
• Product Name: Recombinant Human Programmed Cell Death Protein 1 (PDCD1) Protein (His-SUMO)
• Protein Expression Range: 21-170aa
• Tag: N-6His-SUMO
• Molecular Mass: 32.8kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

PDCD1 is an inhibitory receptor on antigen-activated T-cells that plays a key role in the induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. After T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse, inhibiting T-cell activation. This suppression occurs through the recruitment of PTPN11/SHP-2, which is phosphorylated upon ligand binding, leading to the dephosphorylation of critical TCR signaling molecules, including ZAP70, PRKCQ/PKCtheta, and CD247/CD3zeta.