Recombinant Human Programmed Cell Death Protein 1 (PDCD1) Protein (His), Active

• Purity: Greater than 95% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q15116
• Gene: PDCD1
• CAT.NO: B000222
• Expression Systems: Mammalian cell
• Product Name: Recombinant Human Programmed Cell Death Protein 1 (PDCD1) Protein (His), Active
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Protein Expression Range: 25-167aa
• Tag: C-6His
• Molecular Mass: 18.2 kDa
• Form: Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
• Bioactivity: Active

PDCD1 is an inhibitory receptor on antigen-activated T-cells that plays a crucial role in maintaining immune tolerance to self. It delivers inhibitory signals by binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. After T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse, directly inhibiting T-cell activation. This suppression occurs through the recruitment of PTPN11/SHP-2, which is phosphorylated upon ligand binding. PTPN11/SHP-2 then dephosphorylates key TCR signaling molecules, such as ZAP70, PRKCQ/PKCtheta, and CD247/CD3zeta, reducing T-cell activation.