Recombinant Human Peroxisome Proliferator-Activated Receptor Alpha (PPARA) Protein (His&Myc)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q07869
• Gene: PPARA
• CAT.NO: B000210
• Expression Systems: Baculovirus
• Product Name: Recombinant Human Peroxisome Proliferator-Activated Receptor Alpha (PPARA) Protein (His&Myc)
• Protein Expression Range: 1-468aa
• Tag: N-10His&C-Myc
• Molecular Mass: 56.2 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

This ligand-activated transcription factor is a key regulator of lipid metabolism. It is activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) and oleylethanolamide, a naturally occurring lipid that regulates satiety. The receptor also responds to peroxisome proliferators like hypolipidemic drugs and fatty acids, regulating the peroxisomal beta-oxidation pathway of fatty acids. It activates transcription of the ACOX1 and P450 genes, requiring heterodimerization with RXRA for transactivation, which is antagonized by NR2C2. Additionally, it may play a role in propagating clock information to metabolic pathways regulated by PER2.