Recombinant Human Isocitrate Dehydrogenase [NADP] Cytoplasmic is produced by our Mammalian expression system and the target gene encoding Met1-Leu414 is expressed with a 6His tag at the C-terminus.
| Product Name | Recombinant Human IDH1 Protein (C-6His,Mammalian) |
| Accession | O75874 |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Host Species | Human |
| Gene | IDH1 |
| Source | Human Cells |
| Protein Expression Range | Met1-Leu414 |
| Tag | C-6His |
| Molecular Mass | 47.7 KDa |
| Buffer | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij-35, 10% Glycerol, 1mM DTT, pH8.0. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Bioactivity | Measured by the ability to oxidatively decarboxylate DL-Isocitric acid(Adamas, Catalog #011340133) to 2-oxoglutarate. The specific activity is 2832 pmol/min/μg, as measured under the described conditions. |
