Recombinant Human Hypoxia-Inducible Factor 1-Alpha (HIF1A) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: Q16665
• Gene: HIF1A
• CAT.NO: B000211
• Expression Systems: E.coli
• Product Name: Recombinant Human Hypoxia-Inducible Factor 1-Alpha (HIF1A) Protein (His)
• Protein Expression Range: 579-826aa
• Tag: N-6His
• Molecular Mass: 31.0kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

This protein serves as a master transcriptional regulator of the adaptive response to hypoxia. Under low oxygen conditions, it activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, and HILPDA, among others, which help increase oxygen delivery and facilitate metabolic adaptation. It is essential in embryonic vascularization, tumor angiogenesis, and ischemic disease pathophysiology. It heterodimerizes with ARNT, binding to the hypoxia response element (HRE) in target gene promoters. Its activation involves transcriptional coactivators like CREBBP and EP300 and is enhanced by interactions with NCOA1, NCOA2, and APEX1, influencing mitochondrial distribution in neurons during hypoxia.