Recombinant Human Corticotropin-Releasing Factor Receptor 1 (CRHR1) Protein (His)

• Purity: Greater than 90% as determined by SDS-PAGE.
• Host Species: Homo sapiens (Human)
• Accession: P34998
• Gene: CRHR1
• CAT.NO: B000235
• Expression Systems: Yeast
• Product Name: Recombinant Human Corticotropin-Releasing Factor Receptor 1 (CRHR1) Protein (His)
• Protein Expression Range: 23-121aa
• Tag: N-6His
• Molecular Mass: 13.0kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

This G-protein coupled receptor binds with high affinity to CRH (corticotropin-releasing factor) and UCN (urocortin). Ligand binding induces a conformational change that activates signaling through guanine nucleotide-binding proteins (G proteins) and downstream effectors, including adenylate cyclase. This activation boosts intracellular cAMP levels. The receptor also inhibits the activity of the calcium channel CACNA1H. It is essential for normal embryonic development of the adrenal gland and proper hormonal responses to stress. Additionally, it plays a critical role in the body’s response to anxiogenic stimuli, influencing stress and anxiety regulation.