Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4), partial

• Purity: >85% (SDS-PAGE)
• Host Species: Homo sapiens (Human)
• Accession: Q13639
• Gene: HTR4
• CAT.NO: B000257
• Expression Systems: E.coli In Vivo Biotinylation in E.coli Yeast Baculovirus Mammalian cell
• Product Name: Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4), partial
• Source: E.coli;In Vivo Biotinylation in E.coli;Yeast;Baculovirus;Mammalian cell
• Tag: Tag type will be determined during the manufacturing process.
• Form: Lyophilized powder
• Buffer: Tris/PBS-based buffer, 6% Trehalose.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Alternative Names: HTR4; 5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4
• Protein Length: Partial
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Shelf Life: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

The 5-hydroxytryptamine (serotonin) receptor is a G-protein coupled receptor (GPCR) that binds serotonin, a biogenic hormone functioning as a neurotransmitter, hormone, and mitogen. Upon ligand binding, the receptor undergoes a conformational change, activating guanine nucleotide-binding proteins (G proteins) and modulating downstream effectors. HTR4 is specifically coupled to G(s) G alpha proteins, leading to the activation of adenylate cyclase and subsequent increases in cyclic AMP (cAMP) levels. This signaling pathway plays a role in regulating various physiological processes, including mood, gastrointestinal function, and cell proliferation, through the modulation of cAMP-dependent cellular responses.