Recombinant Clostridium Botulinum Neurotoxin Type A (BOTA) Protein (His), partial

  • Purity: Greater than 90% as determined by SDS-PAGE.
  • Host Species: Clostridium botulinum
  • Accession: P0DPI0
  • Gene: botA
  • CAT.NO: B000279
  • Expression Systems:

    Yeast

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Product Name Recombinant Clostridium Botulinum Neurotoxin Type A (BOTA) Protein (His), partial
Accession P0DPI0
Purity Greater than 90% as determined by SDS-PAGE.
Host Species Clostridium botulinum
Gene botA
Source Yeast
Protein Expression Range 1-436aa
Tag

N-terminal 6xHis-tagged

Molecular Mass 52.0kDa
Form Liquid or Lyophilized powder
Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Storage

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Alternative Names

botA; atx; bonT; Botulinum neurotoxin type A; BoNT/A; Bontoxilysin-A; BOTOX; Botulinum neurotoxin type A1)

Target Protein Sequence MPFVNKQFNYKDPVNGVDIAYIKIPNVGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLLCVRGIIT
Protein Length Partial
Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

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