Biotinylated Recombinant Streptococcus Pneumoniae Penicillin-Binding Protein 2X (PBPX) Protein (MBP&His-Avi)

• Purity: Greater than 85% as determined by SDS-PAGE.
• Host Species: Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
• Accession: P59676
• Gene: PBPX
• CAT.NO: B000231
• Expression Systems: E.coli
• Product Name: Biotinylated Recombinant Streptococcus Pneumoniae Penicillin-Binding Protein 2X (PBPX) Protein (MBP&His-Avi)
• Protein Expression Range: 287-611aa
• Tag: N-MBP&C-6His-Avi
• Molecular Mass: 83.2 kDa
• Form: Liquid or Lyophilized powder
• Buffer: Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Storage: 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.

A transpeptidase, this enzyme forms peptide cross-links between adjacent glycan strands in the cell wall peptidoglycan (PG), contributing to cell wall integrity. It is an essential component of the divisome machinery, involved in synthesizing the septal cross wall during cell division. Beta-lactam antibiotics target and inactivate these penicillin-binding proteins (PBPs) by acylating a crucial serine residue in their active sites. This inhibition disrupts the formation of the bacterial cell wall, leading to cell lysis and preventing bacterial growth, making PBPs important targets for antibiotic therapy.