ZnAF-1

• CAT Number: I045013
• CAS Number: 321859-09-0
• Molecular Formula: C34H28N4O5
• Molecular Weight: 572.61
• Purity: ≥95%
• Synonyms: 6-[2-[bis(pyridin-2-ylmethyl)amino]ethylamino]-3′,6′-dihydroxyspiro[2-benzofuran-3,9′-xanthene]-1-one
• IUPAC Name: 5-[2-[bis(pyridin-2-ylmethyl)amino]ethylamino]-2-(3-hydroxy-6-oxoxanthen-9-yl)benzoic acid
• InChI: InChI=1S/C34H28N4O5/c39-25-8-11-28-31(18-25)43-32-19-26(40)9-12-29(32)33(28)27-10-7-22(17-30(27)34(41)42)37-15-16-38(20-23-5-1-3-13-35-23)21-24-6-2-4-14-36-24/h1-14,17-19,37,39H,15-16,20-21H2,(H,41,42)
• InChIKey: SAVLVCGHDLKAAF-UHFFFAOYSA-N
• SMILES: C1=CC=NC(=C1)CN(CCNC2=CC(=C(C=C2)C3=C4C=CC(=O)C=C4OC5=C3C=CC(=C5)O)C(=O)O)CC6=CC=CC=N6

ZnAF-1（Cat No.:I045013）is a fluorescent probe specifically designed for the selective detection of intracellular zinc ions (Zn²⁺). Featuring a fluorescein-based fluorophore, ZnAF-1 exhibits a marked increase in fluorescence upon binding to free Zn²⁺, enabling real-time imaging of zinc dynamics in live cells. It offers high selectivity over other metal ions, low cytotoxicity, and excellent sensitivity, making it suitable for studying zinc signaling, homeostasis, and transport. ZnAF-1 is widely used in neuroscience, cell biology, and metalloproteomics to explore the roles of zinc in synaptic transmission, apoptosis, and various physiological and pathological processes.