Z-LEVD-FMK

• CAT Number: I042499
• CAS Number: 1135688-25-3
• Molecular Formula: C31H45FN4O10
• Molecular Weight: 652.71
• Purity: ≥95%
• Synonyms: methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-[[(2S)-4-methyl-2-(phenylmethoxycarbonylamino)pentanoyl]amino]-5-oxopentanoate
• InChI: InChI=1S/C31H45FN4O10/c1-18(2)14-23(35-31(43)46-17-20-10-8-7-9-11-20)29(41)33-21(12-13-25(38)44-5)28(40)36-27(19(3)4)30(42)34-22(24(37)16-32)15-26(39)45-6/h7-11,18-19,21-23,27H,12-17H2,1-6H3,(H,33,41)(H,34,42)(H,35,43)(H,36,40)/t21-,22-,23-,27-/m0/s1
• InChIKey: WGJLXPZGHLANRB-FAWUNYRSSA-N
• SMILES: CC(C)CC(C(=O)NC(CCC(=O)OC)C(=O)NC(C(C)C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

Z-LEVD-FMK (Cat No.: I042499) is a synthetic tetrapeptide inhibitor that selectively targets caspase-4, a cysteine protease involved in inflammation and apoptosis. The compound consists of a peptide sequence (Z-Leu-Glu-Val-Asp) conjugated with a fluoromethyl ketone (FMK) moiety, which irreversibly binds to the active site of the enzyme, inhibiting its function. Z-LEVD-FMK is widely used in cell biology research to study caspase-dependent signaling pathways, particularly in pyroptosis and immune responses. Its specificity makes it valuable for dissecting the role of caspase-4 in disease models.

Reference

[1]. Bian ZM, et al. Dual involvement of caspase-4 in inflammatory and ER stress-induced apoptotic responses in human retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 2009 Dec;50(12):6006-14.
[Content Brief]

[2]. Ariazi EA, et al. Estrogen induces apoptosis in estrogen deprivation-resistant breast cancer through stress responses as identified by global gene expression across time. Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):18879-86.
[Content Brief]