Z-Gly-Pro-AMC

• CAT Number: I043055
• CAS Number: 68542-93-8
• Molecular Formula: C25H25N3O6
• Molecular Weight: 463.48
• Purity: ≥95%
• Synonyms: benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate
• InChI: InChI=1S/C25H25N3O6/c1-16-12-23(30)34-21-13-18(9-10-19(16)21)27-24(31)20-8-5-11-28(20)22(29)14-26-25(32)33-15-17-6-3-2-4-7-17/h2-4,6-7,9-10,12-13,20H,5,8,11,14-15H2,1H3,(H,26,32)(H,27,31)/t20-/m0/s1
• InChIKey: YWOXKKRKNGWXEG-FQEVSTJZSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@@H]3CCCN3C(=O)CNC(=O)OCC4=CC=CC=C4

Z-Gly-Pro-AMC（Cat No.:I043055）is a synthetic peptide substrate commonly used in biochemical research, particularly for enzyme activity assays. It consists of the amino acids glycine (Gly) and proline (Pro) attached to a 7-amino-4-methylcoumarin (AMC) fluorophore. The “Z” in the name refers to the benzyloxycarbonyl group, which protects the N-terminus of the peptide. Z-Gly-Pro-AMC is often utilized to measure the activity of prolyl oligopeptidase and other proteases, as the cleavage of the peptide bond releases the AMC fluorophore, producing a detectable fluorescence signal. It is valuable in studying enzymatic processes and screening for enzyme inhibitors.