Z-DEVD-AFC

• CAT Number: I043954
• CAS Number: 1135416-11-3
• Molecular Formula: C36H38F3N5O14
• Molecular Weight: 821.71
• Purity: ≥95%
• Synonyms: (4S)-5-[[(2S)-1-[[(2S)-3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-[[(2S)-3-carboxy-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoic acid
• InChI: InChI=1S/C36H38F3N5O14/c1-17(2)30(34(55)42-23(14-27(47)48)32(53)40-19-8-9-20-21(36(37,38)39)13-29(51)58-25(20)12-19)44-31(52)22(10-11-26(45)46)41-33(54)24(15-28(49)50)43-35(56)57-16-18-6-4-3-5-7-18/h3-9,12-13,17,22-24,30H,10-11,14-16H2,1-2H3,(H,40,53)(H,41,54)(H,42,55)(H,43,56)(H,44,52)(H,45,46)(H,47,48)(H,49,50)/t22-,23-,24-,30-/m0/s1
• InChIKey: JEPURZRNABGOCW-CQUCHYGKSA-N
• SMILES: CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)OCC3=CC=CC=C3

Z-DEVD-AFC（Cat No.:I043954）is a fluorogenic peptide substrate specifically designed to detect caspase-3 and caspase-7 activity, key executioner enzymes in the apoptosis pathway. It consists of the DEVD recognition sequence linked to 7-amino-4-trifluoromethylcoumarin (AFC), a fluorescent reporter. Upon cleavage by active caspases, AFC is released, emitting measurable fluorescence proportional to enzyme activity. Z-DEVD-AFC is widely used in cell death and drug screening assays to monitor apoptosis in real time. Its high specificity and sensitivity make it an essential tool for studying programmed cell death and evaluating apoptosis-targeted therapies in cancer and other diseases.