WST-3

• CAT Number: R000825
• CAS Number: 515111-36-1
• Molecular Formula: C19H10IN6NaO10S2
• Molecular Weight: 696.34
• Purity: ≥95%
• Synonyms: sodium;4-[2-(2,4-dinitrophenyl)-3-(4-iodophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
• InChI: InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30;/h1-10H,(H-,31,32,33,34,35,36);/q;+1/p-1
• InChIKey: LKVNWZJLLRMMAS-UHFFFAOYSA-M
• SMILES: C1=CC(=CC=C1N2N=C(N=[N+]2C3=C(C=C(C=C3)[N+](=O)[O-])[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-])I.[Na+]

WST-3 (Cat No.:R000825) is a colorimetric reagent used in cell viability and cytotoxicity assays. It is reduced by cellular dehydrogenases in metabolically active cells to form a soluble formazan dye, allowing easy quantification of cell proliferation or viability through absorbance measurements. Unlike MTT, WST-3 does not require cell lysis or solubilization steps, offering a simpler, non-toxic, and highly sensitive alternative. Commonly used in drug screening, toxicology, and cell biology, WST-3 enables real-time monitoring of cellular metabolic activity in a variety of research and biomedical applications.