TRFS-red

• CAT Number: I037477
• Molecular Formula: C28H28ClN3O11S2
• Molecular Weight: 682.12
• Purity: ≥95%
• Synonyms: [5-(dithiolan-4-yloxycarbonylamino)benzo[a]phenoxazin-9-ylidene]-bis(3-methoxy-3-oxopropyl)azanium;perchlorate
• InChI: InChI=1S/C28H27N3O7S2.ClHO4/c1-35-25(32)9-11-31(12-10-26(33)36-2)17-7-8-21-23(13-17)38-24-14-22(30-28(34)37-18-15-39-40-16-18)19-5-3-4-6-20(19)27(24)29-21;2-1(3,4)5/h3-8,13-14,18H,9-12,15-16H2,1-2H3;(H,2,3,4,5)
• InChIKey: SGZAVMKJSBHPCL-UHFFFAOYSA-N
• SMILES: COC(=O)CC[N+](=C1C=CC2=NC3=C(C=C(C4=CC=CC=C43)NC(=O)OC5CSSC5)OC2=C1)CCC(=O)OC.[O-]Cl(=O)(=O)=O

TRFS-red（Cat No.:I037477）is a red-emitting, thiol-reactive fluorescent probe tailored for selective detection of hydrogen sulfide (H₂S) in biological environments. Upon reacting with H₂S, TRFS-red undergoes a chemical transformation that triggers a strong red fluorescence signal, enabling high-contrast imaging in live cells and deep tissues. Its red-shifted emission reduces background autofluorescence and enhances compatibility with multi-color imaging. TRFS-red is especially valuable for tracking H₂S signaling pathways involved in oxidative stress, inflammation, and neuromodulation. Its specificity, sensitivity, and bioavailability make it ideal for real-time monitoring of H₂S in complex biological systems.