TPEN - CAS 16858-02-9

TPEN is a specific cell-permeable heavy metal chelator.
Target:
InVitro: Heavy metal chelator TPEN attenuates fura-2 fluorescence changes induced by cadmium, mercury and methylmercury. TPEN, a cell-permeable chelator for heavy metal cations with a low affinity for Ca2+. In cells stimulated with 10 or 30 μM cadmium chloride, the addition of TPEN at 3 hr after exposure significantly decreases the elevated fura-2 fluorescence ratio to the basal levels within 10 min (119.6±2.4% or 109±1.5% decrease in ΔRatio (F340/F380) induced by 10 or 30 μM cadmium chloride, respectively), suggesting that a cadmium chloride-induced increase in the fura-2 fluorescence ratio is dependent on an increase in intracellular heavy metal cations but not intracellular Ca2+[1]. TPEN is a metal chelator, which targets colon cancer cells through redox cycling of copper. TPEN reduces cell viability in a dose- and time-dependent manner. TPEN-induced cell death is also dependent on the redox cycling of copper since the copper chelator neocuproine inhibited DNA damage and reduced pChk1, γ-H2AX, and ATM protein expression. Cell death by low TPEN concentrations, involved ATM/ATR signaling in all 3 cell lines, since pre-incubation with specific inhibitors of ATM and DNA-PK led to the recovery of cells from TPEN-induced DNA damage[2].

Catalog Number: I002201

CAS Number: 16858-02-9

PubChem Substance ID:355036816

Molecular Formula: C26H28N6

Molecular Weight:424.6

Purity: ≥95%

* For research use only. Not for human or veterinary use.

Synonym

SynonymsN1,N1,N2,N2-tetrakis(pyridin-2-ylmethyl)ethane-1,2-diamine

Property

Molecular Formula: C26H28N6
Molecular Weight424.6
Target:Reagents
Solubility10 mM in DMSO
Purity≥95%
StorageStore at +4C

Computed Descriptor

InChIInChI=1S/C26H28N6/c1-5-13-27-23(9-1)19-31(20-24-10-2-6-14-28-24)17-18-32(21-25-11-3-7-15-29-25)22-26-12-4-8-16-30-26/h1-16H,17-22H2
InChIKeyCVRXLMUYFMERMJ-UHFFFAOYSA-N
SMILESc1ccc(nc1)CN(CCN(Cc1ccccn1)Cc1ccccn1)Cc1ccccn1