Thermolysin, Bacillus thermoproteolyticus rokko

• CAT Number: I042552
• CAS Number: 9073-78-3
• Purity: ≥95%

Thermolysin from Bacillus thermoproteolyticus Rokko (Cat No.: I042552) is a thermostable, zinc-dependent metalloproteinase that catalyzes the hydrolysis of peptide bonds, preferentially at the N-terminal side of hydrophobic residues. Known for its high thermal stability and activity at elevated temperatures, it is widely used in protein structure studies, peptide mapping, and industrial applications like peptide synthesis and food processing. Its specificity and robustness make it a valuable tool in biochemical research, particularly for generating protein fragments and modifying peptide sequences under controlled conditions.

Reference

[1]. Bertusvan den Burg, et al. Chapter 111 – Thermolysin and Related Bacillus Metallopeptidases.

[2]. Mu-Forster C, et al. Surface localization of zein storage proteins in starch granules from maize endosperm. Proteolytic removal by thermolysin and in vitro cross-linking of granule-associated polypeptides. Plant Physiol. 1998 Apr;116(4):1563-71.
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[3]. Di Bernardini R, et al. Isolation, purification and characterization of antioxidant peptidic fractions from a bovine liver sarcoplasmic protein thermolysin hydrolyzate. Peptides. 2011 Feb;32(2):388-400.
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[4]. Fujita H, et al: a prodrug-type ACE-inhibitory peptide derived from fish protein. Immunopharmacology. 1999 Oct 15;44(1-2):123-7.
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[5]. Ke Q, et al. Safety evaluation of a thermolysin enzyme produced from Geobacillus stearothermophilus. Food Chem Toxicol. 2013 Sep;59:541-8.
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