TCO-PEG12-NHS ester

• CAT Number: I016618
• CAS Number: 2185016-39-9
• Molecular Formula: C40H70N2O18
• Molecular Weight: 866.99
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[[(4Z)-cyclooct-4-en-1-yl]oxycarbonylamino]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
• InChI: InChI=1S/C40H70N2O18/c43-37-8-9-38(44)42(37)60-39(45)10-12-47-14-16-49-18-20-51-22-24-53-26-28-55-30-32-57-34-35-58-33-31-56-29-27-54-25-23-52-21-19-50-17-15-48-13-11-41-40(46)59-36-6-4-2-1-3-5-7-36/h1-2,36H,3-35H2,(H,41,46)/b2-1-
• InChIKey: NVOXZBGFZZFRHC-UPHRSURJSA-N
• SMILES: C1CC=CCCC(C1)OC(=O)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(=O)ON2C(=O)CCC2=O

TCO-PEG12-NHS ester(CAT: I016618) is a heterobifunctional crosslinker combining a trans-cyclooctene (TCO) group with an N-hydroxysuccinimide (NHS) ester, separated by a PEG12 spacer. The NHS ester enables efficient conjugation to primary amines on proteins, peptides, or other biomolecules, while the TCO group participates in rapid and bioorthogonal inverse electron-demand Diels–Alder (IEDDA) click reactions with tetrazines. The PEG12 chain enhances solubility, reduces steric hindrance, and provides flexible spacing for high-performance labeling or drug delivery systems. This reagent is widely applied in bioconjugation, imaging, targeted therapy development, and advanced biomedical material research where precise and stable covalent modifications are essential.