Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium

• CAT Number: M112916
• CAS Number: 127062-22-0
• Molecular Formula: C20H29N4NaO9S2
• Molecular Weight: 556.59
• Purity: ≥95%
• Synonyms: sodium;2,5-dioxo-1-[6-[5-(2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)pentanoylamino]hexanoyloxy]pyrrolidine-3-sulfonate
• InChI: InChI=1S/C20H30N4O9S2.Na/c25-15(7-4-3-6-13-18-12(11-34-13)22-20(29)23-18)21-9-5-1-2-8-17(27)33-24-16(26)10-14(19(24)28)35(30,31)32;/h12-14,18H,1-11H2,(H,21,25)(H2,22,23,29)(H,30,31,32);/q;+1/p-1
• InChIKey: JJGWLCLUQNFDIS-UHFFFAOYSA-M
• SMILES: C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]

Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium(CAT: M112916) (commonly known as Sulfo-NHS-LC-Biotin) is a water-soluble, amine-reactive biotinylation reagent used for labeling cell surface proteins without membrane permeabilization. It features an NHS ester for covalent coupling to primary amines (e.g., lysine residues) and a spacer arm containing a biotin moiety linked via a 6-carbon chain for enhanced accessibility and minimal steric hindrance. This reagent enables efficient and specific biotin tagging of membrane proteins, facilitating downstream applications such as affinity purification, Western blotting, and flow cytometry. Sulfo-NHS-LC-Biotin is ideal for use in live-cell labeling, protein-protein interaction studies, and the development of biotin-based detection systems.

Reference

Sulfosuccinimidyl-6-(biotinamido)hexanoate sodium (commonly known as Sulfo-NHS-LC-Biotin) is a water-soluble, amine-reactive biotinylation reagent used for labeling cell surface proteins without membrane permeabilization. It features an NHS ester for covalent coupling to primary amines (e.g., lysine residues) and a spacer arm containing a biotin moiety linked via a 6-carbon chain for enhanced accessibility and minimal steric hindrance. This reagent enables efficient and specific biotin tagging of membrane proteins, facilitating downstream applications such as affinity purification, Western blotting, and flow cytometry. Sulfo-NHS-LC-Biotin is ideal for use in live-cell labeling, protein-protein interaction studies, and the development of biotin-based detection systems.