Suc-Ala-Phe-Lys-AMC

• CAT Number: I040409
• CAS Number: 73207-91-7
• Molecular Formula: C32H39N5O8
• Molecular Weight: 621.68
• Purity: ≥95%
• Synonyms: 4-[[(2S)-1-[[(2S)-1-[[(2S)-6-amino-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxohexan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid
• InChI: InChI=1S/C32H39N5O8/c1-19-16-29(41)45-26-18-22(11-12-23(19)26)35-31(43)24(10-6-7-15-33)36-32(44)25(17-21-8-4-3-5-9-21)37-30(42)20(2)34-27(38)13-14-28(39)40/h3-5,8-9,11-12,16,18,20,24-25H,6-7,10,13-15,17,33H2,1-2H3,(H,34,38)(H,35,43)(H,36,44)(H,37,42)(H,39,40)/t20-,24-,25-/m0/s1
• InChIKey: GAQKKPHZOUJFMZ-OPXMRZJTSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCCN)NC(=O)C(CC3=CC=CC=C3)NC(=O)C(C)NC(=O)CCC(=O)O

Suc-Ala-Phe-Lys-AMC(CAT: I040409) is a fluorogenic peptide substrate commonly used to assess the enzymatic activity of trypsin-like serine proteases, especially in proteasome and lysosomal research. The peptide sequence (Ala-Phe-Lys) is cleaved at the lysine residue, releasing the 7-amino-4-methylcoumarin (AMC) fluorophore, which emits fluorescence upon hydrolysis. This allows sensitive and quantitative monitoring of protease activity in biochemical assays. The N-terminal succinyl (Suc) group enhances substrate specificity and stability. Ideal for use in high-throughput screening, inhibitor profiling, and enzyme kinetics, Suc-Ala-Phe-Lys-AMC is supplied at high purity for reliable application in cell biology, drug discovery, and protease research workflows.