For research use only. Not for therapeutic Use.
Suc-AAPF-AMC (CAT: I040630) is a fluorogenic peptide substrate commonly used to measure chymotrypsin-like protease activity, particularly that of the proteasome’s β5 subunit. Upon enzymatic cleavage at the phenylalanine residue, the AMC (7-amido-4-methylcoumarin) moiety is released, producing a measurable fluorescent signal. This substrate is widely used in high-throughput screening, enzymology, and inhibitor profiling to assess proteasomal function and to evaluate the efficacy of protease inhibitors. Its sensitivity, specificity, and compatibility with kinetic fluorescence assays make it a valuable tool in cancer, neurodegeneration, and cell biology research.
| CAS Number | 88467-45-2 |
| Synonyms | 4-[[(2S)-1-[[(2S)-1-[(2S)-2-[[(2S)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxo-3-phenylpropan-2-yl]carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid |
| Molecular Formula | C34H39N5O9 |
| Purity | ≥95% |
| IUPAC Name | 4-[[(2S)-1-[[(2S)-1-[(2S)-2-[[(2S)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxo-3-phenylpropan-2-yl]carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoic acid |
| InChI | InChI=1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-32(45)25(17-22-8-5-4-6-9-22)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,37,45)(H,38,46)(H,41,42)/t20-,21-,25-,26-/m0/s1 |
| InChIKey | FMVUZZZUYBXQGR-KCXKOMAXSA-N |
| SMILES | CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC3=CC=CC=C3)NC(=O)C4CCCN4C(=O)C(C)NC(=O)C(C)NC(=O)CCC(=O)O |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
