SPOP-IN-6b

• CAT Number: I017414
• CAS Number: 2136270-20-5
• Molecular Formula: C28H32N6O3
• Molecular Weight: 500.59
• Purity: ≥95%
• Synonyms: 6-imino-11-methyl-N-(3-morpholin-4-ylpropyl)-2-oxo-7-(2-phenylethyl)-1,7,9-triazatricyclo[8.4.0.03,8]tetradeca-3(8),4,9,11,13-pentaene-5-carboxamide
• InChI: InChI=1S/C28H32N6O3/c1-20-7-5-13-34-25(20)31-26-23(28(34)36)19-22(24(29)33(26)14-10-21-8-3-2-4-9-21)27(35)30-11-6-12-32-15-17-37-18-16-32/h2-5,7-9,13,19,29H,6,10-12,14-18H2,1H3,(H,30,35)
• InChIKey: RYTGHNMOSCSWJT-UHFFFAOYSA-N
• SMILES: CC1=CC=CN2C1=NC3=C(C2=O)C=C(C(=N)N3CCC4=CC=CC=C4)C(=O)NCCCN5CCOCC5

SPOP-IN-6b(CAT: I017414) is a potent speckle-type POZ protein (SPOP) inhibitor with an IC50 of 3.58 μM (patent CN 107141287, SPOP-B-88). It exhibits antiproliferative activity in renal cancer cell lines, showing IC50 values of 2–10.2 μM across A498, Caki-2, Ketr-3, 769-P, OS-RC-2, and 786-0 cells. In cellular studies, SPOP-IN-6b (0.1–3.0 μM, 10 h) suppresses SPOP activity, thereby stabilizing tumor suppressor proteins such as PTEN and DUSP7. In vivo, daily intraperitoneal administration (40–80 mg/kg, 25 days) significantly slows tumor growth through SPOP inhibition, making it a valuable tool compound for oncology and cancer biology research.