• CAT Number: I009569
  • CAS Number: 869998-49-2
  • Molecular Formula: C32H32F3N3O2
  • Molecular Weight: 547.622
  • Purity: ≥95%
Inquiry Now

SPD-304 (CAT: I009569) is an inhibitor of tumor necrosis factor α (TNFα), specifically targeting its binding to the TNF receptor 1 (TNFR1). With an IC50 of 22 µM, SPD-304 effectively blocks the interaction between TNFα and TNFR1. TNFα is a cytokine involved in inflammatory processes and immune responses. The inhibition of TNFα signaling by SPD-304 may have implications in the treatment of conditions characterized by excessive TNFα activity, such as inflammatory disorders. Additionally, both TNFα and the angiotensin II/angiotensin II receptor type 1 (AT1) axis have been implicated in neuropathic pain and nociception, suggesting a potential role for SPD-304 in modulating these processes.

Catalog Number I009569
CAS Number 869998-49-2

SPD-304, SPD 304, SPD304.;6,7-dimethyl-3-[[methyl[2-[methyl[[1-[3-(trifluoromethyl)phenyl]-1H-indol-3-yl]methyl]amino]ethyl]amino]methyl]-4H-1-benzopyran-4-one

Molecular Formula


Purity 95%
Target TNFα inhibitor
Solubility Soluble in DMSO
Storage -20°C
IUPAC Name 6,7-dimethyl-3-[[methyl-[2-[methyl-[[1-[3-(trifluoromethyl)phenyl]indol-3-yl]methyl]amino]ethyl]amino]methyl]chromen-4-one
InChI InChI=1S/C32H32F3N3O2/c1-21-14-28-30(15-22(21)2)40-20-24(31(28)39)18-37(4)13-12-36(3)17-23-19-38(29-11-6-5-10-27(23)29)26-9-7-8-25(16-26)32(33,34)35/h5-11,14-16,19-20H,12-13,17-18H2,1-4H3

1:Arch Pharm (Weinheim). 2014 Nov;347(11):798-805. doi: 10.1002/ardp.201400198. Epub 2014 Aug 27. Rationally designed less toxic SPD-304 analogs and preliminary evaluation of their TNF inhibitory effects.Alexiou P,Papakyriakou A,Ntougkos E,Papaneophytou CP,Liepouri F,Mettou A,Katsoulis I,Maranti A,Tsiliouka K,Strongilos A,Chaitidou S,Douni E,Kontopidis G,Kollias G,Couladouros E,Eliopoulos E, PMID: 25160057 DOI: 10.1002/ardp.201400198 </br><span>Abstract:</span> SPD-304 was discovered as a promising tumor necrosis factor alpha (TNF) antagonist that promotes dissociation of TNF trimers and therefore blocks the interaction of TNF and its receptor. However, SPD-304 contains a potentially toxic 3-alkylindole moiety, which can be bioactivated to a reactive electrophilic intermediate. A series of SPD-304 analogs was synthesized with the aim to diminish its toxicophore groups while maintaining the binding affinity for TNF. Incorporation of electron-withdrawing substituents at the indole moiety, in conjunction with elimination of the 6/’-methyl group of the 4-chromone moiety, led to a significantly less toxic and equally potent TNF inhibitor.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Request a Quote

Contact Us at MuseChem

We are committed to providing you with reliable, cost-effective solutions for your chemical needs, while ensuring your safety and comfort. Our team of experts is always available to answer your questions and help you navigate the complexities of the chemical industry.

Whether you're looking for a specific product or need help with a custom synthesis project, we're here to help you discover a new world of chemical possibilities. Contact us today to learn more about how we can assist you with all of your chemical needs.

Our goal is to make the process of ordering chemicals as seamless and hassle-free as possible. Let us know how we can assist you, and we'll get back to you as soon as possible. We look forward to hearing from you!